Fig. 3

Effect of Rev-D4F on EPC numbers. a The number of CD34⁺ cells, FLK-1⁺ cells, CD133⁺ cells, CD34⁺/FLK-1⁺ cells, FLK-1⁺ /CD133⁺ cells, and CD34⁺/CD133⁺ cells from mice peripheral blood in NS group as a percentage of the control. Data are presented as mean ± SD (n = 6; **P < 0.01 versus NS, ^#P < 0.05, ^##P < 0.01 versus LPS group). b IHC of Sca-1⁺ cells in the right lobes of the lungs; scale bar represents 50 μm. c Densitometric evaluation of the data shown in panel B. d The FLK-1⁺/CD133⁺ double positive cells were detected by immunofluorescence staining (× 400); scale bar represents 20 μm. e The percentage of CD34⁺/FLK-1⁺ positive cells shown in panel D. f Western blot analysis of Sca-1, FLK-1, and CD133 protein levels in lung tissues. g-i Densitometric evaluation of the western blotting data shown in panel f (*P < 0.05, **P < 0.01)