Fig. 6

Effects of CTHRC1 and FHL2 knockdown in HFL-1 cells. Collagen gel contraction and chemotaxis were assessed in CTHRC1- and FHL2-knocked down HFL-1 cells. Western blot analysis of the effects of CTHRC1 silencing on targets related to fibrotic processes (a). Collagen gel contraction (b) and chemotaxis (c) after silencing of CTHRC1. The effect of CTHRC1 knockdown on TGF-β1 (0.25 ng/mL)-induced gel contraction (d) and chemotaxis toward to fibronectin (e). Western blot analysis to analyze the effects of FHL2 silencing on targets related to fibrotic processes (f). Collagen gel contraction and (g) chemotaxis (h) after silencing of FHL2. Collagen gel contraction, vertical axis: gel size measured after 2 days of contraction expressed as a percentage of the initial value. Chemotaxis, vertical axis: number of migrated cells per 5 HPF. Horizontal axis: conditions. Values represent means ± SEMs of three separate experiments. *P < 0.05, **P < 0.01, ***P < 0.001