Fig. 2

Effects of pirfenidone on collagen gel contraction and chemotaxis in primary lung fibroblasts. Fibroblasts from controls and patients with pulmonary fibrosis (PF) were cultured in the presence or absence of pirfenidone (100 μg/mL) and 0.25 ng/mL TGF-β1, and collagen gel contraction and chemotaxis were assayed. (a) Collagen gel contraction. Vertical axis: gel size measured after 2 days of contraction expressed as a percentage of the initial gel area. Horizontal axis: conditions. (b) Pirfenidone-induced suppression of collagen gel contraction in lung fibroblasts isolated from controls and patients with IP. Vertical axis: percentage of gel contracted size following pirfenidone treatment ([difference in gel size/initial gel size] × 100). Horizontal axis: conditions. (c) Fibroblast chemotaxis. Vertical axis: number of migrated fibroblasts per 5 HPF. Horizontal axis: conditions. (d) Pirfenidone-dependent suppression of chemotaxis in lung fibroblasts isolated from controls and patients with IP. Vertical axis: percentage of migrated cells following pirfenidone treatment ([difference in migrated cell number/initial migrated cells number] × 100). Horizontal axis: conditions. Each patient evaluated was expressed as an individual symbol, representing the means of two to three separate experiments, each of which included three replicates. (a, c) Lines connect the values for individual patients in the presence or absence of pirfenidone. *P < 0.05, **P < 0.01, ***P < 0.001. (b, d) Student’s paired t test and unpaired t test were used. *P < 0.05, **P < 0.01, ***P < 0.001