Fig. 4
From: Piezo1 induced apoptosis of type II pneumocytes during ARDS
Piezo1 was a potential mechanism to explain the protective role in lung injury of LTV. a Wet/dry ratio decreased and oxygenation index (calculated as PaO2/FiO2) in different group. Lung morphometry assessed by HE staining (100X/400X magnification) b The apoptosis of type II pneumocytes assayed by flow cytometry. c Immunofluorescence staining of Piezo1 and SP-A. Piezo1 and SP-A double positive cells stained in orange.(100 × magnification) d Ca2+ influx detected by confocal microscope and the quantitation of immunofluorescence presented as a bar graph. (1000 × magnification) e The expression of caspase 3,Bcl-2 and Piezo1 in rats assessed by western blot (cleaved caspase 3 was an activated form of caspase 3) (NS, not significant. *, p < 0.05.**, p < 0.001.LV low tidal volume, HV high tidal volume.) f The relative protein expression levels of cleaved caspase3/caspase3,Bcl-2 and Piezo1