Fig. 3
From: Piezo1 induced apoptosis of type II pneumocytes during ARDS
Piezo1 mediated apoptosis depends on calcium influx. a A549 cells were treated with 15% stretch with a frequency of 0.5HZ for 4 h. Ca2+ was labeled with10 μM Fluo-3/AM and observed by confocal imaging. Selected eight visual fields randomly and detected total fluorescent intensity by Image pro Plus 6.0.Calculatedsingle cell fluorescent intensity as total fluorescent intensity/cell counts. In Ca2+ free group, calcium was removed from medium. (1000 × magnification) b A549 was transfected with Piezo1-siRNA72hrsbefore treated with mechanical stretch and Ca2+ influx was assayed by confocal imaging. (1000 × magnification) c The apoptosis of A549 treated with mechanical stretch was assessed by flow cytometry and annexin V-fluorescein isothiocyanate/PI double staining. **p < 0.01.vs. DMEM +Control siRNA; ##p < 0.01.vs. DMEM+Piezo1 siRNA. d The expression of apoptosis related proteins are assayed by western blot. e The relative protein expression levels of cleaved caspase3/caspase3,Bcl-2 and Piezo1