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Fig. 1 | Respiratory Research

Fig. 1

From: SU5416 does not attenuate early RV angiogenesis in the murine chronic hypoxia PH model

Fig. 1

Effects of SU5416 on primary MCEC in vitro. (a) When exposed to recombinant VEGF (50 ng/mL) in vitro, signaling through VEGFR-2 is rapidly activated, as demonstrated by phosphorylation of VEGFR-2 (Y1175) and ERK. Pre-treatment with 5 μM SU5416 completely inhibits VEGFR-2 Y1175 phosphorylation and subsequent ERK phosphorylation. Graphs show densitometry from n = 4/group. "denotes time in minutes. (b) Pre-treatment with 5 μM SU5416 attenuates recombinant VEGF-induced cell proliferation (n = 3/group). (c) Tube formation on Matrigel is not inhibited by SU5416 (5 μM). Representative images are shown, as are quantitative estimates of total branching length and number of nodes calculated from n = 3 experiments/group. (d) SU5416 pre-treatment (5 μM) inhibits recombinant VEGF mediated increase in Kdr (VEGFR-2) transcription (n = 4/group) 6 h following stimulation. (E) VEGFR-2 protein levels are not increased 18 h after stimulation with recombinant VEGF, and this effect is not modified by SU5416 pre-treatment (5 μM). All error bars represent SEM. * denotes P < 0.05; ** denotes P < 0.01. Additional P-values are for Student’s t-test (C) or one-way ANOVA (d, e)

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