Fig. 3

Vascular effects of Endothelin-1 and imatinib in human pulmonary vessels. a Imatinib in naïve PAs/PVs: (⃝) PA (n = 4); (□) PV (n = 6). b Concentrations-response curve of ET-1 in naïve human pulmonary vessels: (●) PA (n = 7); (■) PV (n = 6). c ET-1 in human PAs/PVs: (●) PA 100 nM ET-1 (n = 4); (■) PV 50 nM ET-1 (n = 6). d Effect of increasing concentration of imatinib in pre-constricted PAs: (⃝) 100 nM ET-1 (n = 7); (●) 100 nM ET-1 / imatinib (n = 10). e Effect of increasing concentration of imatinib in pre-constricted PVs: (□) 50 nM ET-1 (n = 6); (■) 50 nM ET-1 / imatinib (n = 6). f Effect of increasing concentration of imatinib in pre-constricted PVs: (□) 20 nM ET-1 (n = 6); () 20 nM ET-1 / imatinib (n = 4) b, d EC₅₀ values were calculated using the standard 4-paramter logistic non-linear regression model. c) Statistics was performed by a LMM. e, f Statistics was performed by the Mann-Whitney U test. P < 0.05 are considered as significant: ^* p < 0.05, ^** p < 0.01 and ^*** p < 0.001