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Fig. 2 | Respiratory Research

Fig. 2

From: Transmembrane-4 L-six family member-1 (TM4SF1) promotes non-small cell lung cancer proliferation, invasion and chemo-resistance through regulating the DDR1/Akt/ERK-mTOR axis

Fig. 2

The knockdown of TM4SF1 suppressed colony formation and cell invasion and migration in A549 and H1299 cell lines. a RT-PCR and western blots confirmed the silencing of TM4SF1 in A549 and H1299 cell lines. b The silencing of TM4SF1 by siRNA inhibited colony formation in A549 and H1299 cell lines (left panel). Right panel showed quantitative analysis of colony formation. The numbers of colonies in NC cells were set to 100%. Values are expressed as the mean ± SD from three independent experiments, and the asterisk indicates the statistical significance compared to the controls (***, p < 0.001). c Cell migration and invasion assay in NC and TM4SF1 silenced A549 and H1299 cell lines by a 24-transwell system. Original magnification× 10, Asterisks indicate a significant level of proliferation compared with controls (***, p < 0.001), the right panel indicates the quantitative analysis of transwell cell invasion and migration assay

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Respiratory Research

ISSN: 1465-993X

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