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Fig. 3 | Respiratory Research

Fig. 3

From: Tobacco exposure inhibits SPLUNC1-dependent antimicrobial activity

Fig. 3

Tobacco smoke exposure leads to increased H. influenzae colonization. HBECs obtained from normal donors were exposed to air or tobacco smoke from Marlboro cigarettes, Cheyenne little cigars and Swisher Sweets Strawberry little cigars, once a day for 5 days. 20 μl of H. influenzae at 106 CFU/ml were added apically to HBECs for 2 h at 37 °C/5% CO2. a Apical supernatants and (b) lysates were collected, serially diluted, plated on LB agar plates and incubated at 37 °C for 48 h to determine colony-forming units (CFU) for bacterial load (all n = 9). Statistically significant differences were measured using the Kruskal-Wallis test. **p < 0.001, ***p < 0.0001 different to air control

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