Fig. 6

Modulation of superoxide dismutase (Sod) 1 expression through hyperoxia and caffeine. Quantitation of lung homogenates by a) ELISA and b) qPCR of for 3 days’ postnatal oxygen exposure (P3) and recovery (P3_P15), and 5 days’ postnatal oxygen exposure (P5) and recovery (P5_P15), respectively. Data are expressed relative to the normoxia-exposed control group (100%) and the 100% values for ELISA quantitation are 1.5 μg/mg, 1.0 μg/mg, 0.8 μg/mg, and 1.3 μg/mg protein for P3 and P3_P15 or P5 and P5_P15 groups, respectively. Error bars represent SEM, n = 5 (ELISA) or n = 7–8 (pPCR)/group. **p < 0.01 and ***p < 0.001 versus control (NO); #p < 0.05, ##p < 0.01, and ###p < 0.001 versus hyperoxia group (HY) with Mann Whitney test