Fig. 3

Inhibition of LDH5 by Compound 408 does not inhibit FMT in primary human lung fibroblasts. Image based analysis of α-SMA fibres, fibronectin (FN) and the numbers of nuclei in primary human lung fibroblasts treated with LDH5 inhibitor (Compound 408) and TGF-β1 for 72 h. Representative images of stained α-SMA fibres (a). Dose-response curves of the effect of increasing concentrations of LDH5 inhibitor (Compound 408) on nuclei numbers (b), fibronectin (FN) expression relative to untreated and TGF-β1 controls (c), α-SMA expression relative to untreated and TGF-β1 controls (d), and lactate production (e) with dashed lines indicating the mean IC50 for glycolytic capacity and glycolysis in TGF-β1-treated cells. N = 2, n = 8