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Fig. 4 | Respiratory Research

Fig. 4

From: The cullin4A is up-regulated in chronic obstructive pulmonary disease patient and contributes to epithelial-mesenchymal transition in small airway epithelium

Fig. 4

Epithelial-to-mesenchymal transition (EMT) in HSAEpiC cells was induced by CUL4A ectopic expression. a The expression of CUL4A was analysis by western blot in stable transfection of CUL4A HSAEpiC cells. b The proliferation of HSAEpiC cells after CUL4A overexpression was observed by MTT assay. c The proliferation of HSAEpiC cells after CUL4A overexpression was observed by BrdU-ELISA assay. d Changes in morphology of HSAEpiC-CUL4A cells were observed by microscopy. e After overexpression of CUL4A, the expression of epithelial (E-cadherin and α-catenin) and mesenchymal (N-cadherin and Vimentin) marker proteins was examined by Western blotting. f After overexpression of CUL4A, the expression of epithelial (E-cadherin) and mesenchymal (N-cadherin) protein markers was determined by immunofluorescence assay. **P < 0.01 compared to pBabe group. All results are from 3 independent experiments. Error bars indicate standard deviation

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Respiratory Research

ISSN: 1465-993X

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