Fig. 4
From: Salicylic acid amplifies Carbachol-induced bronchoconstriction in human precision-cut lung slices
Salicylic acid or toluene diisocyanate has little effect on agonist-induced [Ca2+]i in HASM cells. Confluent HASM cells were exposed to salicylic acid (SA: 0.1, 1 uM; vehicle: F-12 medium) or toluene diisocyanate (TDI: 0.1, 1 uM; vehicle: 0.1% ethanol) for 24 h, loaded with fluo-8 dye and stimulated with carbachol (CCh, 10 uM) or histamine (1 uM) to determine cytosolic elevation of Ca2+ ([Ca2+]i). SA has little effect on a-c) histamine or b-d) carbachol-induced [Ca2+]i. TDI has little effect on e-g) histamine or f-h) carbachol-induced [Ca2+]i. (Data representative of n = 3 donors. A, B, E, F: baseline-corrected mean fluorescence in relative fluorescence units (RFU); C, D, G, H: mean ± SEM of n = 3 donors, Area Under the Curve (AUC) of the Ca2+ transient; t (s): time in seconds)