Fig. 3

TDI enhances carbachol-induced phosphorylation of myosin light chain in HASM cells. HASM cells were exposed to salicylic acid (SA 1 uM, vehicle F-12 medium) or toluene diisocyanate (TDI 1 uM, vehicle 0.1% ethanol) or dinitrochlorobenzene (DNCB 0.1 uM, vehicle 0.1% ethanol) for 24 h. Baseline or carbachol (CCh)-induced MLC phosphorylation was determined. a-c) TDI (1 uM) significantly decreased the baseline (n = 3 donors, *p = 0.047) MLC phosphorylation, while enhancing carbachol-induced MLC phosphorylation (n = 3 donors, *p = 0.03). d-f) SA or g-i) DNCB had little effect on baseline or carbachol-induced MLC phosphorylation (n = 3 donors)