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Fig. 1 | Respiratory Research

Fig. 1

From: Long non-coding RNA CASC2 suppresses pulmonary artery smooth muscle cell proliferation and phenotypic switch in hypoxia-induced pulmonary hypertension

Fig. 1

Up-regulation of lncRNA CASC2 inhibited proliferation in hypoxia-induced PASMCs. (a) The expression of lncRNA CASC2 was detected by qRT-PCR in PASMCs. (b) Cell number of PASMCs was counted by CyQuant cell proliferation assay kit. (c) The mRNA expression of PCNA was detected by qRT-PCR in PASMCs. (d) The protein expression of PCNA was detected by western blot analysis in PASMCs. (e, f) Cell proliferation status of PASMCs was assessed by EdU staining assay. ###p < 0.001, compared with normoxia group. ## p < 0.01, compared with normoxia group. #p < 0.05, compared with normoxia group. **p < 0.01, *p < 0.05, compared with hypoxia + pcDNA3.1 group

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