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Fig. 5 | Respiratory Research

Fig. 5

From: Role of RASEF hypermethylation in cigarette smoke-induced pulmonary arterial smooth muscle remodeling

Fig. 5

Cigarette smoke influenced RASEF promoter methylation and transcription in rat PAs. Genome-wide DNA methylation sequencing and heat map of rat PAs revealed a number of changes in gene distribution associated with different methylation sites (a, b). c Cigarette smoke caused hypermethylation of a CpG within the RASEF promoter (located at NC_005104.4–90,418,763) in rat PAs. d, e The methylation status of CpGs in RASEF promotor in rat PAs was measured by MassARRAY®. f RASEF mRNA levels were lower in the smoking PH versus control rat PAs. g RASEF immunofluorescence was much lower in the pulmonary arterioles of PH rats versus controls. Data are displayed as the mean ± standard error of the mean (n = 4); *P < 0.05, ***P < 0.001 vs. Air group

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Respiratory Research

ISSN: 1465-993X

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