Fig. 4

Effect of DNMT1 siRNA on CSE-induced HPASMCs proliferation and migration changes. a-c DNMT1 siRNA reduced DNMT1 mRNA and protein expression in HPASMCs. Pretreatment of HPASMCs with DNMT1 siRNA suppressed 48-h CSE-induced increases in cell viability (d) and cell cycle transition from G0/G1 to S or G2/M phase (e, f). g, h DNMT1 siRNA transfection prevented 24-h CSE-induced migration of HPASMCs. Scale bar = 100 μm. Data are displayed as the mean ± standard error of the mean (n = 3); *P < 0.05, **P < 0.01, ***P < 0.001