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Fig. 6 | Respiratory Research

Fig. 6

From: Ethyl pyruvate reduces organic dust-induced airway inflammation by targeting HMGB1-RAGE signaling

Fig. 6

ODE exposure induces ROS and nitrite (secreted RNS) production and EP treatment reduces ODE-induced ROS production. Media alone (control, 0 h) or ODE (6, 24, and 48 h) treated cells were subjected to CM-H2DCFDA and Griess’ assay to quantify intracellular ROS production (a) and secreted nitrite concentration (b) respectively (n = 6). ODE exposure of cells resulted in significant increase in intracellular ROS and nitrite secretion (secreted RNS) into the media as early as 6 h post-treatment. Compared to vehicle treatment, cells co-treated with EP (2.5 μM) showed a significant reduction in ODE-induced ROS production at 48 h. Data analyzed with two-way ANOVA is represented (a and b). ## or **p < 0.01 and **** or #### p < 0.0001. # indicates significantly different from control whereas * indicates significant difference within the OD/barn exposure groups

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