Fig. 13
From: Ethyl pyruvate reduces organic dust-induced airway inflammation by targeting HMGB1-RAGE signaling
Antibody neutralization treatment reduces ODE-induced ROS and nitrite production. Cells or supernatants were subjected to CM-H2DCFDA and Griess’ assay to quantify intracellular ROS production (a) and secreted nitrite concentration (b) respectively (n = 6). Compared to ODE exposure alone, ODE exposed cells co-treated with Anti-HMGB1 antibody (10 μM) showed a significant reduction in ODE-induced ROS production (48 h) and secreted nitrite (6 and 48 h). Data analyzed with two-way ANOVA is represented (a and b). * or # p < 0.05, ** or ## p < 0.01, *** or ### p < 0.001, **** or #### p < 0.0001. # indicates different from control whereas * indicates difference within the OD/barn exposure groups