Fig. 5

Role of PLCε in LPS-induced chemokine production. a The RNA was collected from the primary cultures of PLCε^+/+ and PLCε^∆X/∆X AECs in 16 h after the administration of LPS (500 ng/mL) or vehicle (n = 8 mice/group). The RNAs were pooled from each group and subjected to qRT-PCR. *, p < 0.05 between control and LPS administration, #, p < 0.05 between PLCε genotypes. b Primary cultured lung AECs from PLCε^+/+ and PLCε^∆X/∆X mice were prepared for Cxcl5 (green) immunostaining after stimulation for 90 min by LPS (500 ng/ml) (n = 8 mice/group). The Cxcl5 antibody (15 μg/ml) was used. Nuclei were counter-stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bar, 25 μm