Fig. 1

Pirfenidone reduces bleomycin-induced pulmonary fibrosis in mice. Groups of minimum five mice were used throughout these experiments. Mice were treated with either a saline sterile solution (control) or bleomycin (0.02 U) (Bleo). Bleomycin-treated mice orally received twice daily either 200 mg/kg/dose of pirfenidone resuspended in carboxymethylcellulose (Bleo + Pirf) or carboxymethylcellulose alone (Bleo + CMC). Saline-treated mice received carboxymethylcellulose (control). Mice were sacrificed at day 15 post-bleomycin administration for lymphocyte count in the BAL (a) and hydroxyproline measurement in the lungs (lung HPO) (b). Data are the mean ± SEM. Statistical analyses were performed by an ANOVA one-way test with Tukey’s test for comparisons between groups. **, p < 0.01; ***, p < 0.001