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Fig. 6 | Respiratory Research

Fig. 6

From: Loss of the adhesion G-protein coupled receptor ADGRF5 in mice induces airway inflammation and the expression of CCL2 in lung endothelial cells

Fig. 6

Il4, Il5, Il13, IL-25, and IL-33 are upregulated in the lungs of Adgrf5−/− mice. a–c The mRNA expression of Il4, Il5, and Il13 was analyzed by qPCR in whole (1-week-old) or post-lavage (3, 6, 12, and 30 weeks of age) lungs of WT and Adgrf5−/− mice. The data were normalized to Gapdh levels and are expressed as values relative to those of corresponding WT mice. Values are presented as the mean ± SEM (n = 3–5). *p < 0.05; **p < 0.005; ***p < 0.0001; ND, not detected. d and e Whole lung lysates (20 μg of protein) from WT and Adgrf5−/− mice (3, 5, and 12 weeks of age) were analyzed by western blotting using antibodies specific for IL-25 (d, top), IL-33 (e, top), and α-tubulin (bottom). An asterisk indicates nonspecific bands. The band intensity was normalized to that of α-tubulin and is expressed as a value relative to that in corresponding WT mice. Values are presented as the mean ± SEM (n = 3). *p < 0.05 and **p < 0.01

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