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Fig. 10 | Respiratory Research

Fig. 10

From: Loss of the adhesion G-protein coupled receptor ADGRF5 in mice induces airway inflammation and the expression of CCL2 in lung endothelial cells

Fig. 10

CCL2 treatment does not affect the expression of MUC5AC, SLC26A4, IL-25, or IL-33 in NCI-H292 cells. a The mRNA expression of MUC5AC and SLC26A4 was analyzed by qPCR in NCI-H292 cells treated with or without 100 ng/ml CCL2 for 18 h. The data were normalized to GAPDH levels and are expressed as values relative to those from untreated control cells. Values are presented as the mean ± SEM (n = 3–5). b The protein expression of IL-25 and IL-33 was analyzed by western blotting in NCI-H292 cells treated with or without 100 ng/ml CCL2 for 18 h. Whole cell lysates (20 μg of protein) were analyzed by western blotting using antibodies specific for IL-25 (left top), IL-33 (right top), and α-tubulin (bottom). The expression of IL-25 was not detected. The band intensity of IL-33 was normalized to that of α-tubulin and expressed as a value relative to that from untreated control cells. Values are presented as mean ± SEM (n = 4)

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Respiratory Research

ISSN: 1465-993X

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