Skip to main content
Fig. 6 | RESPIRATORY RESEARCH

Fig. 6

From: LncRNA H19 promotes the proliferation of pulmonary artery smooth muscle cells through AT1R via sponging let-7b in monocrotaline-induced pulmonary arterial hypertension

Fig. 6

PDGF-BB-H19-Let-7b axis regulates AT1R expression. PASMCs were stimulated with PDGF-BB at a concentration of 0, 10 and 100 ng/ml for 48 h. AT1R mRNA (a) and protein (b) were detected by RT-qPCR and Western Blot, respectively. PASMCs were transfected with 1000 ng of vector or pH 19 for 48 h. H19 and AT1R mRNA (c) were detected by RT-qPCR, and AT1R protein (d) was detected by Western Blot. PASMCs were stimulated with PDGF-BB at a concentration of 100 ng/ml for 48 h. Then, PASMCs were transfected with (50 nM siCon+ 150 nM iCon), (50 nM siH19 + 150 nM iCon) or (50 nM siH19 + 150 nM iLet-7b) for 24 h, respectively. H19 and AT1R mRNA (e) were detected by RT-qPCR, and AT1R protein (f) was detected by Western Blot. Values were presented as means ± SD from 3 independent experiments. *0.01 ≤ P ≤ 0.05 (different from the corresponding control); **0.001 ≤ P ≤ 0.009 (different from the corresponding control); ***P < 0.001 (different from the corresponding control); #0.01 ≤ P ≤ 0.05 [(siH19 + iLet-7b) vs. (siH19 + iCon)]; ###P < 0.001 [(siH19 + iLet-7b) vs. (siH19 + iCon)]

Back to article page