Fig. 1

H19 is upregulated in rat lungs following MCT treatment and is induced by cytokines in vitro. a: At 3 weeks after injecting rats with MCT (60 mg/kg) or saline (control), the right ventricular systolic pressure (RVSP) was measured. b: Summary of representative RVSP measurements. c: Representative microphotographs of pulmonary small arteries. Lung sections stained with haematoxylin-Eosin (H&E). Scale bar = 50 μm. d: The ratio of right ventricle to left ventricle plus septum weight RV/ (LV + S). e: Expression of H19 in the lung or serum of MCT-induced rats and C57/BL6 mice (WT mice). f: Expression of IL-1α, IL-1β, IL-6, PDGF-AA, PDGF-BB, TNF-α and VEGF in the lungs of MCT-induced rats and WT mice. g: Expression of H19 in PASMCs stimulated by cytokines at a concentration of 20 ng/ml for 48 h. h: Expression of H19 in PASMCs stimulated with IL-1β and PDGF-BB at a concentration of 10 and 100 ng/ml for 48 h. Values were presented as means ± SD (n = 6 in each group). *0.01 ≤ P ≤ 0.05 (different from the corresponding control); **0.001 ≤ P ≤ 0.009 (different from the corresponding control); ***P < 0.001 (different from the corresponding control); ##0.001 ≤ P ≤ 0.009 (IL-1β-100 vs. IL-1β-10); &0.001 ≤ P ≤ 0.009 (PDGF-BB-100 vs. PDGF-BB-10)