Fig. 2

VASP phosphorylation, profilin1 and cofilin1 expression in human lung autopsy tissue. Lung tissues sections were obtained from infants that died with BPD (closed squares) or infants that died at similar ages from non-pulmonary illnesses (open circles). Fluorescent microscopy followed by NIH Image J analysis was used to quantify VASP expression and differential phosphorylation, and profilin1 and cofilin1 expression in human autopsy samples. Images are 40X magnification. Green, VASP, phosphor-VASP, profilin1 and cofilin1 stained with specific antibody against each protein; red, F-actin stained with phalloidin; and blue, nuclei stained with DAPI. Four independent images were obtained from each slide and averaged for each individual creating n = 8 for each group. Statistical analysis was performed using Welch’s unequal variances t-test, n = 8 in each group, and significance is indicated as *, p < 0.05; and n.s. = non significant compared to respective controls