Fig. 3
From: MUC1 deficiency mediates corticosteroid resistance in chronic obstructive pulmonary disease
Cigarette smoke mediates corticosteroid resistance and decreases MUC1 expression in human neutrophils. a Cigarette smoke extract (CSE) decreased the mRNA gene expression of MUC1 in sputum neutrophils from smokers. b-e Peripheral blood neutrophils from healthy and COPD patients were stimulated with (b, d) lipopolysaccharide (LPS) or (c, e) with cigarette smoke extract (CSE) during 6 h, and IL-8 and MMP9 levels were measured by ELISA. f-i Peripheral blood neutrophils form healthy, smokers and COPD patients were incubated with dexamethasone for 1 h and stimulated with LPS or CSE for 6 h. The results are shown as mean ± SE of n = 4 cell populations from healthy subjects, smokers and COPD patients. Gene expression was analyzed by real time PCR using the 2-ΔCt as described in methods. Two-way ANOVA followed by Bonferroni post-hoc tests. a-e *P < 0.05 compared with control/basal; #P < 0.05 compared with healthy group. f-i *P < 0.05 compared with healthy group; #P < 0.05 compared with smoker group