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Fig. 7 | Respiratory Research

Fig. 7

From: Unfractionated heparin ameliorates pulmonary microvascular endothelial barrier dysfunction via microtubule stabilization in acute lung injury

Fig. 7

Effect of UFH or SB203580 pretreatment on LPS-induced disassembly of MTs. HPMECs were pretreated with UFH (10 U/ml) or SB203580 (10 μM) for 30 min, followed by LPS (10 μg/ml) stimulation for 6 h. a MT remodeling was examined by immunofluorescence staining with Alexa Fluor® 488, (scale bars = 10 μm). UFH and SB203580 prevented LPS-induced MT disassembly. b Western blotting was used to detect polymeric/monomeric tubulin. UFH and SB203580 prevented LPS-induced MT disassembly. c Acetylated tubulin was examined by immunofluorescence staining with cyanine 2, (scale bars = 10 μm). UFH and SB203580 enhanced acetylated tubulin expression. d Pool of stable MTs was determined by western blot analysis with antibodies against acetylated tubulin. UFH and SB203580 enhanced acetylated tubulin levels. Data are representative of three independent experiments and are shown as means ± SD. t-test, * P < 0.05 compared to the vehicle group. t-test, # P < 0.05 compared to the LPS-treated group

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