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Fig. 1 | Respiratory Research

Fig. 1

From: Unfractionated heparin ameliorates pulmonary microvascular endothelial barrier dysfunction via microtubule stabilization in acute lung injury

Fig. 1

UFH ameliorates LPS-induced endothelial barrier dysfunction by inhibiting MT disassembly and dynamics of GEF-H1 in vivo. C57BL/6 J mice were challenged with LPS (30 mg/kg, intraperitoneally) for 6 h with or without subcutaneous injection of UFH (8 U, subcutaneously). a HE staining (scale bars = 100 μm). Administration of UFH suppressed LPS-stimulated pulmonary histopathological changes. b Lung W/D and lung Evans blue values. LPS-stimulated group show marked increase in the lung W/D ratio and enhanced pulmonary microvascular leakage. c Western blot analysis was used to evaluate the levels of acetylated tubulin, GEF-H1, and MYPT1 phosphorylation. Equal protein loading was confirmed using GAPDH. UFH decreased the LPS-stimulated increase in GEF-H1 expression and MYPT1 phosphorylation. Data are presented as means ± SD (n = 3–5 mice per group). t-test, *P < 0.05, compared to vehicle. t-test, # P < 0.05, compared to LPS alone

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