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Fig. 2 | Respiratory Research

Fig. 2

From: Dietary ω-6 polyunsaturated fatty acid arachidonic acid increases inflammation, but inhibits ECM protein expression in COPD

Fig. 2

Similar arachidonic acid-induced COX-2 mRNA expression in COPD and non-COPD. Pulmonary fibroblasts from COPD (n = 5) and non-COPD patients (n = 5) were unstimulated (control) or challenged with ω-6 PUFA arachidonic acid (AA) in 0.1% BSA-DMEM (100 μM) for 6 (a), 9 (b), 24 (c) or 48 (d) hours. Total RNA was extracted and cyclooxygenase (COX)-2 mRNA expression was measured using qPCR. The results are normalized to the endogenous control (18S rRNA), and presented as fold change from control (t = 0 h) ± standard error of the mean. Unpaired t-test was used to determined statistical significance. There were no statistical differences

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