Fig. 7

Single confocal optical section of the airway epithelium in a cryosection of the lungs of a GAD67-GFP mouse 48 h after challenge with LPS. a BrdU-positive nucleus (arrowhead; red Cy3 fluorescence) in the airway epithelium, and some subepithelial BrdU staining (open arrowhead). b Combination with GAD67-GFP fluorescence (green), marking PNECs in the NEB ME. c Image of the three channels. In the same section, CCs/CLCs are immunostained for the Clara cell-specific protein CCSP (blue pseudo-color of Cy5 fluorescence), and are seen to surround GFP-labeled NEB cells. Note that the divided epithelial cell (BrdU-labeled nucleus; arrowhead) is located adjacent to the PNECs and co-stained with CCSP, and can therefore be identified as a CLC. L: airway lumen, E: airway epithelium