Fig. 3
LCI representation of the effects of application of BALF of an LPS-challenged mouse to the NEB ME in a vibratome cut live lung slice of a control mouse. a Graphs plotting the time course of Fluo-4 fluorescence intensity, as an indicator for [Ca2+]i, in different cell types in the airway epithelium. To facilitate interpretation, grey levels of each region of interest (ROI) were adjusted for the basal level of (background) fluorescence at the start of imaging. After application of BALF (=T0), CLCs that surround the NEB cells show a calcium-mediated activation within about 10 s (=T1). The oscillating [Ca2+]i rise in CLCs continues (=T2) for more than a minute post-exposure. No changes in [Ca2+]i can be observed in NEB cells or CCs. b, c Corresponding pseudo-color time-lapse images of Fluo-4 fluorescence at two time points (B = T0; C = T2) after the application of BALF. Note that the ROIs corresponding to the graphs in (a) are represented in the same color code in image b