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Fig. 1 | Respiratory Research

Fig. 1

From: Selective activation and proliferation of a quiescent stem cell population in the neuroepithelial body microenvironment

Fig. 1

Representative images, with similar cell densities, of Diff-Quick-stained cytospin preparations of BALF collected from mice that received no instillation (untreated healthy control animal; a), 16 h after an intratracheal instillation with 0.9% NaCl (sham-treatment; b), or with LPS (c, d). Cell densities of the cytospin preparations were ‘normalized’ between the experimental groups and are therefore unrelated to the initial cell numbers in the BALF. a BALF of a healthy control mouse contains virtually no other cells than macrophages (acentric oval nuclei and bluish cytoplasm). b Some neutrophils (segmented nuclei and unstained cytoplasm; arrowheads) appear to be infiltrated in the airways after a sham instillation but macrophages still constitute the majority of cells in this sample. c, d Massive neutrophil influx is seen as a response to the LPS instillation, and consequently a relatively low number of macrophages (open arrowheads). Note that the preparations of treated animals also harbor some red blood cells (small brownish dots)

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