Fig. 3

Ectopically expressed FBXO17 increases cell proliferation and cell viability. a A549 cells were transfected with FBXO17-V5 plasmid or empty vector. Live cells were counted 48 h after transfection using trypan blue exclusion staining. Data is representative of three independent experiments, **p < 0.001. b A549 cells were transfected with FBXO17-V5 plasmid or empty vector. After 24 h cells were plated at 5 × 10⁴/well in a 96-well plate. MTS was added at 24, 48, and 72 h. After 30 min, samples were analyzed by spectrophotometry at a wavelength of 490 nm. Data is representative of three independent experiments, *p < 0.01, **p < 0.001. c A549 cells were transfected with FBXO17-V5 or empty vector and cultured in complete medium for 24 h. Complete medium was replaced with serum-free medium for 16 h. Cells were then pulsed with BrdU in completed medium for 45 min. After removal of BrdU, cells were cultured in complete medium for an additional 6 h. Cells were processed and stained with anti-BrdU and 7-AAD and analyzed by flow cytometry. Data in (c) is graphically represented for each phase and shown in (d). Data is representative of three independent experiments, *p < 0.01