Fig. 2
From: FBXO17 promotes cell proliferation through activation of Akt in lung adenocarcinoma cells
FBXO17 increases Akt activation through site-specific phosphorylation. a A549 cells were transfected with FBXO17-V5 plasmid or empty vector. Lysates were prepared at 48 h after transfection and immunoblotted for indicated kinases. b Analysis of phosphorylation bands for kinases was quantitated using densitometry and results graphed as shown. Analysis of protein levels and relative phosphorylation were quantitated using densitometry with β-actin as a loading control and results were graphed as shown. Data is representative of three independent experiments, *p < 0.05. c A549 cells were transfected with scrambled siRNA and siFBXO17. Lysates were prepared 72 h after transfection and immunoblotted. Analysis of protein levels and relative phosphorylation were quantitated using densitometry with β-actin as a loading control and results were graphed as shown. Quantitative RT-PCR and immunoblotting were performed to confirm reduced FBXO17 expression after knockdown (below). Data is shown on effect of FBXO17 siRNA on steady-state FBXO17 mRNA levels. Data is representative of three independent experiments, *p < 0.05