Skip to main content
Fig. 5 | Respiratory Research

Fig. 5

From: Upregulation of microRNA-17-5p contributes to hypoxia-induced proliferation in human pulmonary artery smooth muscle cells through modulation of p21 and PTEN

Fig. 5

Hypoxia reduces the expression of miR-17-5p predicted targets. PASMCs were exposed to normoxic or hypoxic conditions for 48 h. a mRNA levels of the genes involved in proliferation and apoptosis in PASMCs under hypoxic or normoxic conditions. b Hypoxia reduces the protein expression of validated miR-17-5p targets, including PTEN and p21. c When miR-17-5p was inhibited, PASMC proliferation was significantly decreased under both normoxia and hypoxia. The results are expressed as the means ± SD of at least three independent experiments, and qRT-PCR was conducted in triplicate. d PCNA expression was examined by western blot analysis and normalized to GAPDH expression. *P < 0.05, #P < 0.01

Back to article page

Respiratory Research

ISSN: 1465-993X

Contact us