Fig. 2

miR-17-5p plays a role in PASMC proliferation. PASMCs were transfected with an miR-17-5p agomir (miR-17-5p), an miR-17-5p inhibitor (anti-miR-17-5p), or a negative control (NC). a miR-17-5p expression. The results are the means ± SD, and the experiments were performed in triplicate and were repeated a minimum of three times. b Overexpression of miR-17-5p increased cell proliferation. The results are the means ± SD of three independent experiments, and in each experiment, three parallel samples were analysed per group. c Protein level of PCNA. The representative blots are shown in the upper panel, and the quantification is shown in the lower panel. The results are the means ± SD of three separate experiments. d Inhibition of miR-17-5p reduced PASMC proliferation. The data are shown as the means ± SD of three independent experiments, and in each experiment, three parallel samples were analysed per group. e The expression of PCNA was analysed by western blot analysis and normalized to GAPDH expression. The data are expressed as the means ± SD (n = 4). *P < 0.05, #P < 0.01