Fig. 1

Purification of the amino terminal of PrtA. a The PrtA fragment (amino acids 144–1041) containing the catalytic triad was cloned and expressed. b Illustration of the PrtA fragment used for expression and purification. c PrtA1 and PrtA2 purified using Ni-NTA resin alone or with glutathione-Sepharose 4B were resolved by SDS-polyacrylamide gel electrophoresis and visualized by Coomassie Brilliant Blue staining