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Fig. 3 | Respiratory Research

Fig. 3

From: Differential effects of Nintedanib and Pirfenidone on lung alveolar epithelial cell function in ex vivo murine and human lung tissue cultures of pulmonary fibrosis

Fig. 3

Effect of in vitro treatment with Pirfenidone and Nintedanib on primary mouse (pm)ATII cells. a, b At day 14 after Bleomycin instillation, mice were sacrificed and control (PBS) and fibrotic (Bleo) pmATII cells were harvested. The pmATII cells were cultured in the presence of Nintedanib (1 μM) and Pirfenidone (500 μM) for 48 h. a Gene expression analysis by qPCR of fibrotic marker Fn1 in pmATII cells. ΔCp relative to Hprt is presented as mean ± SEM, n = 3. Means were compared using repeated-measures one-way ANOVA followed by Newmann-Keuls post test. b Gene expression analysis by qPCR of epithelial cell markers Sftpc, Nkx2.1, T1α, Hopx. ΔCp relative to Hprt is presented as mean ± SEM, n = 3. Means were compared using repeated-measures one-way ANOVA followed by Newmann-Keuls post test. Significance: *p < 0.05, **p < 0.01, ***p < 0.001 (DMSO vs Pirfenidone/Nintedanib). Significance: #p < 0.05, ##p < 0.01, ###p < 0.001 (PBS vs Bleo)

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