Fig. 7
Effects of MRS2578 on production of cytokines/chemokines in two-hit lung injury. Mice were intranasally instilled with P. aeruginosa (1× 106 CFU) or PBS and received MRS2578 or vehicle (DMSO) 15 min, 24 h and 48 h after pneumonia, and then subjected to mechanical ventilation with either low (LP) or high (HP) pressure for 2 h. The bronchoalveolar lavage fluid (BALF) was collected 24 h after mechanical ventilation. The concentrations of 6 cytokines/chemokines were simultaneously determined by Luminex multiplex assays. N = 7 per group in P. aeruginosa groups, N = 5 per group in PBS groups. * p < 0.05 vs. FVB at identical conditions analyzed by Holm-Sidak test; † p < 0.05 vs. LP at identical conditions analyzed by Tukey’s test; ¶ p < 0.05 vs. PBS at the identical conditions analyzed by Tukey’s test; # p < 0.05 vs. DMSO at identical conditions analyzed by Tukey’s test, respectively