Fig. 4

Effects of MRS2578 in HNP⁺ mice with pneumonia followed by mechanical ventilation. HNP⁺ mice were intranasally instilled with P. aeruginosa (1× 10⁶ CFU) and then subjected to keep spontaneous breathing (SB) or mechanical ventilation with high pressure (HP) for 2 h. MRS2578 at 10 μM or 20 μM was administrated intraperitoneally 15 min, 24 h and 48 h after pneumonia. Total cell counts (a) and neutrophil counts (b) in bronchoalveolar lavage fluid (BALF) were determined. c Lung histology (H&E) was obtained 72 h after bacterial instillation. Neutrophil infiltration (circle) was indicated. N = 7 per group. * p < 0.05 vs. spontaneous breathing (SB) at identical conditions analyzed by Holm-Sidak test; † p < 0.05 vs. DMSO at identical conditions analyzed by Tukey’s test, respectively