Fig. 3
Effects of MRS2578 in pneumonia. Mice were intranasally instilled with P. aeruginosa (1× 106 CFU) or PBS. a HNP levels in bronchoalveolar lavage fluid (BALF) were measured 72 h later. In additional mice, MRS2578 or vehicle (DMSO) was administered intraperitoneally 15 min, 24 h and 48 h after bacterial instillation. Bacteria counts (b) and neutrophils (c) in the BALF were determined 72 h after bacterial instillation. d Lung histology (H&E) was obtained 72 h after bacterial instillation, Pulmonary edema (black arrow) and neutrophil infiltration (circle) were indicated. e Lung permeability was determined by albumin concentration in BALF. f Lung injury score. N = 7 per group in P. aeruginosa groups, N = 5 per group in PBS groups. * p < 0.05 vs. FVB at identical conditions analyzed by Holm-Sidak test; ¶ p < 0.05 vs. PBS at the identical conditions analyzed by Tukey’s test; # p < 0.05 vs. DMSO at identical conditions analyzed by Tukey’s test, respectively