Fig. 1

HNP binding to BEAS-2B cells through P2Y receptor family. BEAS-2B cells were incubated with FITC-HNP for 30 min (a). HNP are labeled in green and F-actin in red. HNP binding was inhibited by addition of Reactive Blue (RB), or Suramin (b and c) 30 min prior to FITC-HNP. Effect of HNP on IL-8 production. HNP (100 μg/mL) induce IL-8 production by BEAS-2B cells in a time-dependent manner (d). RB (10 μM) or suramin (10 μM) added 30 min prior to HNP administration attenuated IL-8 production in BEAS-2B cells (e) and human THP-1 monocytes (f) at 8 h. * p < 0.05 vs. control group analyzed by Holm-Sidak test; † p < 0.05 vs. HNP group at other three time points analyzed by Tukey’s test; ¶ p < 0.05 vs. HNP group analyzed by one-way ANOVA, respectively