Transforming growth factor beta1 targets estrogen receptor signaling in bronchial epithelial cells

Respiratory Research

Table 2 Primer information for qPCR

Gene	Forward (5′-3′)	Reverse (5′-3′)	Protocol	Efficiency	Source
GAPDH	GAAGGTGAAGGTCGGAGTC	GAAGATGGTGATGGGATTTC	95C 3m; 95C 10s, 60C 30s, ×40	93.9%	[92]
ESR1	CCACCAACCAGTGCACCATT	GGTCTTTTCGTATCCCACCTTTC	95C 3m; 95C 10s, 60C 30s, ×40	100.7%	[93]
ESR2	Proprietary	Proprietary	95C 3m; 95C 10s, 60C 30s, ×40	102.9%	Bio-Rad
GPER	GCTCCCTGCAAGCAGTCTTT	GAAGGTCTCCCCGAGAAAGC	95C 3m; 95C 10s, 60C 30s, ×40	97.2%	[94]
SNAI1	CCAGACCCACTCAGATGTCAA	GGACTCTTGGTGCTTGTGGA	95C 3m; 95C 10s, 58C 30s, ×40	97.2%	[94]
CDH1	GAAAGCGGCTGATACTGACC	CTCAGACTAGCAGCTTCGGA	95C 3m; 95C 10s, 58C 30s, ×40	104.9%	[94]
ACTA2	CATCATGCGTCTGGATCTGG	GGACAATCTCACGCTCAGCA	95C 3m; 95C 10s, 60C 30s, ×40	94.8%	[95]
MMP2	TGTGTTCTTTGCAGGGAATG	TCCAGAATTTGTCTCCAGCA	95C 3m; 95C 10s, 58C 30s, ×40	93.6%	[96]
CTGF	AATGCTGCGAGGAGTGGGT	CGGCTCTAATCATAGTTGGGTCT	95C 3m; 95C 10s, 60C 30s, ×40	96.4%	[97]
VIM	GCGTGAAATGGAAGAGAACT	GGTATCAACCAGAGGGAGTG	95C 3m; 95C 10s, 56C 10s, 72C 30S, ×40	104.0%	[30]
MUC15	CCATCGGCGACTTTATGACG	TCTTCACTTTCTGGCATGGCT	95C 3m; 95C 10s, 60C 30s, ×40	92.1%	[94]

ISSN: 1465-993X