Skip to main content
Fig. 7 | Respiratory Research

Fig. 7

From: Transforming growth factor beta1 targets estrogen receptor signaling in bronchial epithelial cells

Fig. 7

Orthogonal validation of RNA-Seq data. a-d Expression of select genes was validated by qPCR; ESR1 (a), Connective tissue growth factor (CTGF, b), VIM (c), and Matrix metalloproteinase 2 (MMP2, d), in an identical and independent experiment. Bars represent expression [Log2(Fold Change)] of each gene in the RNA-Seq analysis, and black dots represent expression [Log2(Fold Change)] in each sample (n = 6) in the orthogonal experiment as determined by qPCR relative to vehicle control (DMSO). Target gene expression as measured by qPCR was normalized to GAPDH mRNA expression and quantified as fold change to control using the relative ΔΔCq method. Asterisks (*) indicate differential expression compared to controls [Log2(Fold Change) ≥ |0.6| and FDR-corrected p-value < 0.05] in the RNA-Seq analysis, and pound signs (#) indicate statistically significant (p < 0.05) differences compared to vehicle controls in the qPCR data as determined by one-way ANOVA and Newman-Keuls multiple comparison test

Back to article page