Fig. 6

TGF-β1 and E2 exhibit distinct transcriptional profiles. a BEAS-2Bs were exposed to 5 ng/mL TGF-β1 and 10 nM E2 individually and in combination. Cells were acclimated for 24 h, then groups 2 and 3 were exposed to TGF-β1. After 24 h, groups 3 and 4 were exposed to E2, and all samples were collected 24 h thereafter. b Venn diagram highlighting distribution of differentially expressed genes [Log2(Fold Change) ≥ |0.6| and FDR-corrected p-value < 0.05] among the treatment groups. c Heat map showing the clustering and relative expression levels [Log2(Fold Change) compared to controls] of genes that were differentially expressed in at least one treatment group. Red coloring indicates up-regulation compared to controls and green coloring indicates down-regulation compared to controls, (T, TGF-β1; T + E, TGF-β1 + E2; E, E2)