Fig. 2

E2 does not significantly affect TGF-β1-induced EMT. BEAS-2B cells were exposed to 5 ng/mL TGF-β1 in the presence or absence of 10 nM E2 for 48 h. (a-f) Expression of CDH1 (a), VIM (b), ACTA2 (c), SNAI1 (d), CDH2 (e), and FN1 (f) mRNA was measured by qPCR. Target gene expression was normalized to GAPDH mRNA expression and quantified as fold change to control using the relative ΔΔCq method. Data are mean ± SEM of three or four (a-d) or two (e-f) independent experiments. Different letters indicate statistically significant (p < 0.05) differences between groups as determined by one-way ANOVA and Newman-Keuls multiple comparison test