Fig. 1

TGF-β1 induces EMT-like changes in mRNA expression in BEAS-2B cells. (a-f) BEAS-2B cells were exposed to TGF-β1 (0.1, 1, and 5 ng/mL) for 48 h and mRNA expression of the epithelial cell type marker, E-cadherin (CDH1, a), the mesenchymal cell type markers Vimentin (VIM, b), Snail family transcriptional repressor 1 (SNAI1, d), Cadherin 2 (CDH2, e), and Fibronectin (FN1, f), and the myofibroblast cell type marker, Alpha smooth muscle actin (ACTA2) was measured by qPCR. Target gene expression was normalized to GAPDH mRNA expression and quantified as fold change to control using the relative ΔΔCq method. Data are mean ± SEM of three or four independent experiments. Different letters indicate statistically significant (p < 0.05) differences between groups as determined by one-way ANOVA and Newman-Keuls multiple comparison test