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Fig. 7 | Respiratory Research

Fig. 7

From: Regulatory role of heme oxygenase-1 in silica-induced lung injury

Fig. 7

Effect of HO-1 byproducts on silica-induced pERK and HO-1 upregulation in RAW264.7 and 16HBE cells. Immunoblotting analysis showed that HO-1 byproducts, bilirubin and CO, suppress silica-induced ERK activation and subsequent HO-1 upregulation in (a) RAW264.7 cells and (b) 16HBE cells. Cells were pretreated with bilirubin or RuCO (CO releasing molecule) 1 h before silica exposure at the indicated concentrations and incubated as described in Fig. 4. Representative immunoblot images from three independent experiments are shown. Densitometric analysis of band intensity representing the mean ± SD level of pERK to ERK or HO-1 to actin from three independent experiments. # P < 0.05; ## P < 0.01 vs silica only

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