Fig. 3
From: Regulatory role of heme oxygenase-1 in silica-induced lung injury
Effect of ketoconazole on HO-1 induction and ERK activation in the lungs in murine silicosis. Mice were administered intraperitoneally with the HO-1 selective inhibitor ketoconazole (KTZ) at 48, 24, and 0.5 h before 2.5 mg of silica particles instillation. a Lung samples collected 2 days after silica instillation were analyzed as described in Fig. 1. b Densitometric analysis of band intensity representing the mean ± SD level of HO-1 protein relative to actin (n = 3/group). The ratio of HO-1/actin was significantly decreased in the lungs in murine silicosis pretreated with KTZ compared to the no pretreatment group (silica alone). c Lung samples collected 2 days after silica instillation were analyzed as described in Fig. 2. d Densitometric analysis of band intensity representing the mean ± SD level of pERK to ERK (n = 3/group). The ratio of pERK/ERK was significantly increased in the lungs in murine silicosis pretreated with KTZ compared to the no pretreatment group (silica alone). *, P < 0.05; **, P < 0.01